This study examines the treatment motivation, stigma, and disclosure in 287 HIV-infected patients in Pakistan. Four validation scales were applied to assess treatment motivation, stigma and disclosure of patients. The results showed that female patients had significantly higher levels of stigma and disclosure than male patients. Unmarried patients had significantly higher levels of stigma and disclosure than married patients. Moreover, the treatment motivation significantly positively correlated with self-disclosure. Stigma significantly positively correlated with disclosure. Stigma and self-disclose could predicate the treatment motivation. The study concluded that female and unmarried patients with HIV positive had more stigma and disclosure. Stigma, and disclosure affect the treatment motivation of patients with HIV positive