The present study aimed to examine the role of alexithymia and emotional regulation in dissociative tendencies among university students. Moreover, it also focused to determine the moderating role of emotional regulation in predicting dissociative tendencies. Sample (N = 250) included both boys (n = 125) and girls (n = 125) from public and private sector universities with age ranged from 18-25 years. Measures of Toronto Alexithymia Scale (Taylor, 1994), Emotion Regulation Questionnaire (Gross, & John, 2003) and Dissociative Tendencies Questionnaire (Bernstein & Putnam, 1986) were used to assess the study variables. Results showed positive association among alexithymia, expressive suppression (emotional regulation component) and dissociative tendencies. Findings further showed negative association of alexithymia, expressive suppression and dissociative tendencies with cognitive reappraisal (emotional regulation component). In addition, cognitive reappraisal component of emotional regulation buffered the relationship between alexithymia and dissociative tendencies; while, expressive suppression (emotional regulation component) antagonized the relationship between alexithymia and dissociative tendencies. Significant gender differences were found with boys displayed more alexithymia, expressive suppression, and dissociative tendencies. On the contrary, girls expressed better cognitive reappraisal. Implications, limitations and suggestions for future researches had also been discussed.